目的：探讨在体条件下，非创伤性双下肢缺血后处理是否具有减轻心肌缺血/再灌注损伤（IR）的作用。方法：采用SD大鼠心肌缺血/再灌注模型，应用Evan’s蓝、TTC染色，观察非创伤性双下肢缺血后处理对心梗面积(infarction size IS)的影响；并应用生化检测及免疫组化技术，研究非创伤性双下肢缺血后处理磷酸肌酸激酶（creatine kinase CK）、丙二醛（malondialehyde MDA）、超氧化物歧化酶（superoxide dismutase SOD）对大鼠心肌缺血/再灌注损伤的作用。结果：与缺血/再灌注组比较，非创伤性双下肢缺血后处理组的IS、MDA含量显著降低( P<0.01)，而SOD含量明显升高(P< 0.01)，而CK值无显著性差异(P >0.05)。结论：非创伤性双下肢缺血后处理能有效降低心肌缺血/再灌注梗塞面积,其机制可能与减少自由基损伤和抗氧化作用加强有关。

关键词： 缺血 预处理 再灌注 心肌梗塞

【Abstract】Objective: To observe protective effects of non-wound legs ischemic postconditioning on ischemia/reperfusion myocardial injury in rats, investigate the effects of postconditioning on ischemia/reperfusion myocardial injury. Methods: 30 Sprague-Dawley rats divided randomly into four groups and reproduce the model of ischemia/reperfusion myocardial injury. We measured the infarction sizes (IS) with Evan’s and TTC dye, examine creatine kinase （CK）, malondialehyde （MDA）and superoxide dismutase （SOD） in our study. Results: Compare with the ischemic reperfusion group, the IS and the concentration of MDA in plasma of non-wound legs ischemic postconditioning were lower, p<0.01, and the concentration of SOD in plasma of non-wound legs ischemic postconditioning were higher than that of the ischemic reperfusion group. Compare with the ischemic reperfusion group, there are no significance in ischemic reperfusion group and non-wound legs ischemic postconditioning group, p>0.05. Compare with the ischemic preconditioning group,

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